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The Rapid Whole Blood DNA Mini Kit is designed for fast and efficient purification of genomic DNA from fresh or frozen whole blood samples. The optimized spin-column technology enables researchers to obtain high-quality DNA within approximately 8 minutes, making it ideal for PCR, qPCR, sequencing, genotyping, and molecular diagnostics applications.
✓ Process 200–600 μL Whole Blood
✓ Fresh or Frozen Blood Samples
✓ No Proteinase K Required
✓ High Yield and High Purity
✓ PCR, qPCR and Sequencing Ready
✓ Efficient Removal of Hemoglobin and PCR Inhibitors
Ultra-Fast 8-Minute Protocol
This premium whole blood DNA extraction kit utilizes specialized spin columns that require only one spin and one wash, cutting hands-on processing time to under 8 minutes.
No Erythrocyte Lysis Buffer Needed:
Bypasses the tedious and messy red blood cell lysis steps entirely, minimizing sample loss during blood genomic DNA purification.
Enzyme-Free Workflow
Delivers a rapid, incubation-free protocol without requiring Proteinase K digestion or ethanol addition, streamlining your column-based blood DNA prep.
Innovative 3-in-1 Lysis Buffer
A proprietary molecular biology reagent that single-handedly lyses anticoagulated blood, releases high-yield genomic DNA, and precipitates hemoglobin simultaneously.
Highly Versatile Sample Compatibility
Perfectly optimized for 300–600 μl of fresh or frozen whole blood, as well as challenging specimens like bone marrow (EDTA/heparin anticoagulated)
The Rapid Whole Blood DNA Extraction Mini Kit represents the next generation of spin column-based DNA isolation technology, specifically engineered for robust total DNA extraction from blood.
Traditional methods for whole blood genomic DNA isolation often struggle with low purity and persistent cellular contaminants. This kit solves those industry bottlenecks by introducing an advanced one-step lysis and hemoglobin precipitation chemistry. After a brief centrifugation, the DNA-bearing supernatant is loaded directly onto a high-affinity silica-membrane spin column. The high-yield genomic DNA binds selectively to the matrix, while cellular debris, residual proteins, and notorious PCR inhibitors are efficiently washed away.
| Feature | Conventional Kit | Rapid Whole Blood DNA Mini Kit |
| Processing Time | 30–90 min | 8 min |
| Proteinase K Digestion | Required | Not Required |
| Phenol-Chloroform | Sometimes | No |
| PCR Compatibility | Yes | Yes |
| Fresh Blood | Yes | Yes |
| Frozen Blood | Limited | Yes |
| Bone Marrow Samples | Limited | Yes |
Rapid Whole Blood DNA Mini Kit Cat. No. | 50 Preps FD3003050 | 250 Preps DF3003250 |
Spin Columns 2 ml Collection Tubes Buffer L7 Buffer WB (Concentrate) Buffer TE Instructions | 50 50 35 ml 19 ml 12 ml 1 | 250 250 160 ml 50 ml×2 60 ml 1 |
Step 1. Blood Lysis
Whole blood cells are lysed to release genomic DNA.
Step 2. Hemoglobin Removal
Hemoglobin and cellular contaminants are efficiently removed.
Step 3. DNA Binding
Genomic DNA selectively binds to the silica membrane.
Step 4. Column Washing
Residual proteins, salts, and inhibitors are removed.
Step 5. DNA Elution
High-quality purified genomic DNA is recovered.
The kit delivers high DNA recovery and excellent purity across a wide range of blood input volumes.
| Blood Volume | Typical DNA Yield | A260/A280 |
| 50 μL | 4–6 μg | 1.78–1.85 |
| 100 μL | 7–10 μg | 1.80–1.86 |
| 200 μL | 10–15 μg | 1.80–1.88 |
| 600 μL | 15–20 μg | 1.82–1.90 |
Figure 2. DNA yield and purity obtained from different whole blood input volumes.
Purified genomic DNA was evaluated using standard PCR amplification assays.
Strong amplification bands were observed across all tested blood samples, demonstrating excellent compatibility with downstream PCR applications.
Figure 3. PCR amplification results using genomic DNA purified with the Rapid Whole Blood DNA Mini Kit.
Figure 4. Common downstream applications of genomic DNA purified from whole blood samples.
The data below highlights the excellent performance and consistency of this blood genomic DNA purification kit across different sample volumes (300\ 600ul) using normal human blood samples (processed in duplicate). All extracted genomic DNA samples were eluted in a final volume of100ul TE Buffer.
Table 1: Spectrophotometric Analysis of Extracted Genomic DNA
Sample No. | Sample Volume (μl) | OD260 | OD280 | OD230 | OD260/OD230 | OD260/OD280 | Concentration (ng/μl) |
1 | 300 | 1.371 | 0.761 | 0.711 | 1.93 | 1.8 | 67.96 |
2 | 300 | 1.534 | 0.858 | 0.685 | 2.24 | 1.79 | 76.69 |
3 | 600 | 2.259 | 1.266 | 1.18 | 1.91 | 1.79 | 112.97 |
4 | 600 | 2.376 | 1.346 | 1.315 | 1.81 | 1.77 | 118.81 |
Figure 1: Agarose Gel Electrophoresis
Agarose gel electrophoresis of high-molecular-weight genomic DNA extracted from normal human blood samples using the Rapid Whole Blood DNA Extraction Mini Kit. Lane M: Forest DL15000 DNA Ladder; Lanes 1–2: Genomic DNA extracted from 300 μl blood samples; Lanes 3–4: Genomic DNA extracted from 600 μl blood samples (5 μl sample loaded per lane).
(Image Description: 5 μl of each extracted genomic DNA sample loaded on a 1% agarose gel, demonstrating intact, high-molecular-weight gDNA bands with zero signs of RNA or protein contamination.)
(Image Description: 18 μl of eluted DNA template used in a 40 μl PCR amplification system. The strong, clear bands prove that all PCR inhibitors were thoroughly eliminated, confirming the kit's superiority in nucleic acid purification.)
Lane 5: Negative Control.
Lane 6: Positive Control.
Lane M: Forest DL2000 DNA Ladder.
Target Gene: Human B-globin gene (approx. 1.3 kb fragment).
Product Name | Cat. No. | Pack Size | Storage |
Rapid Whole Blood DNA Extraction Mini Kit | FD3003050 | 50 Preps | Store at 0–30°C |
Rapid Whole Blood DNA Extraction Mini Kit | FSD3003250 | 250 Preps | Store at 0–30°C |
Shelf Life：24 Months
Answer: This kit offers highly versatile sample compatibility. It is perfectly optimized for 200–600 μL of fresh or frozen whole blood, as well as challenging specimens such as anticoulated bone marrow. It supports common anticoagulants including EDTA, Heparin, and Citrate without affecting
downstream purity.
Answer: Thanks to our proprietary Innovative 3-in-1 Lysis Buffer, the molecular biology reagent single-handedly lyses blood cells, releases high-yield genomic DNA, and precipitates hemoglobin simultaneously. This enzyme-free workflow eliminates the need for time-consuming Proteinase K incubation or messy erythrocyte (RBC) lysis steps, completing the column-based blood DNA prep in just 8 minutes.
Answer: Hemoglobin is a notorious PCR inhibitor in blood DNA extraction. Our specialized spin column chemistry features an advanced Hemoglobin Removal stage. During the initial lysis, the 3-in-1 buffer chemically precipitates the bulk of heme/hemoglobin, ensuring that only pure genomic DNA binds to the silica membrane. This guarantees an exceptionally high $A_{260}/A_{280}$ ratio (typically 1.7–1.9) and leaves the eluted DNA completely free of PCR inhibitors.
Answer: Yes, absolutely. Because the protocol bypasses harsh enzymatic digestions and avoids ethanol precipitation artifacts, the eluted genomic DNA maintains excellent structural integrity with high yield. It is fully PCR, qPCR, and NGS Library Preparation ready, ensuring unbiased coverage and high-quality sequencing scores.
Answer:
Kit Storage: The entire kit (including the proprietary 3-in-1 Lysis Buffer and columns) should be stored at room temperature (15–30°C). It remains stable for up to 24 months under these conditions.
Purified DNA Storage: For short-term downstream applications, store the eluted DNA at 4°C. For long-term preservation, we recommend storing the genomic DNA aliquots at -20°C or -80°C to prevent degradation
8-Minute Extraction Workflow
