Canine Health Screening Panel (5-Plex) qPCR Kit | Dog Pathogen PCR

The Canine Health Screening Panel (5-Plex) qPCR Kit(FVPD503) is a multiplex molecular detection kit designed for the detection of five important canine pathogen targets associated with infectious disease research and veterinary health screening.

The panel detects:

Canine Parvovirus (CPV)
Canine Coronavirus (CCoV)
Canine Distemper Virus (CDV)
Canine Adenovirus Type II (CAV-2)
Mycoplasma

The kit uses a rapid real-time fluorescence PCR method and is supplied in a lyophilized PCR reagent format for convenient laboratory testing.

For Veterinary Research Use Only.

The product specification is 10 Tests/Kit, with storage at 0–20°C and a stated shelf life of 12 months.

H2 — Five-Pathogen Canine PCR Panel

The Canine Health Screening Panel (5-Plex) qPCR Kit combines five canine pathogen targets into one molecular testing panel.

H3 — Detection Targets

The product instructions specify that the kit is intended for the detection of nucleic acids from these five canine pathogen targets.

H2 — What Does the Canine 5-Plex PCR Kit Detect?

H3 — Canine Parvovirus (CPV)

Canine Parvovirus is an important pathogen investigated in canine infectious disease research. Molecular detection of CPV nucleic acid can support laboratory research involving canine infectious diseases.

H3 — Canine Coronavirus (CCoV)

The panel includes a target for Canine Coronavirus, allowing laboratories to include coronavirus testing within a broader canine pathogen screening workflow.

H3 — Canine Distemper Virus (CDV)

The kit includes Canine Distemper Virus as one of the five molecular detection targets.

H3 — Canine Adenovirus Type II (CAV-2)

The panel includes Canine Adenovirus Type II as specified in the current product instructions.

H3 — Mycoplasma

Mycoplasma is included as the fifth pathogen target in this canine molecular screening panel.

H2 — Why Choose a Canine 5-Plex PCR Panel?

A multi-target PCR panel can provide laboratories with a broader molecular screening approach than a single-pathogen PCR assay.

The Canine Health Screening Panel combines:

CPV + CCoV + CDV + CAV-2 + Mycoplasma

into one five-target testing solution.

H3 — Key Advantages

• 5 canine      pathogen targets in one PCR panel

• Rapid      real-time fluorescence PCR

• Lyophilized      PCR reagent format

• Multiple      canine swab sample types

• Simple sample      preparation workflow

• Room-temperature      storage at 0–20°C

• 12-month      shelf life

• 10 tests per      kit

The current instructions specify the lyophilized format, 10-test package, 0–20°C storage and 12-month shelf life.

H2 — Sample Types

The Canine Health Screening Panel is designed for canine swab samples collected according to the product instructions.

H3 — Anal Swab

Collect an anal swab sample using a new sterile flocked swab.

H3 — Oral Swab

Collect a canine oral swab sample using a new sterile flocked swab.

H3 — Nasal Swab

Collect a nasal swab sample using a new sterile flocked swab.

H3 — Ocular Swab

Collect a sample from the ocular mucosa using a new sterile flocked swab.

The instructions specifically describe anal swabs and oral, nasal and ocular swab samples as sample types.

H2 — Rapid Sample Preparation Workflow

The Canine Health Screening Panel uses a simplified sample preparation workflow before real-time fluorescence PCR detection.

H3 — Step 1: Sample Collection

Collect the appropriate canine swab sample using a new flocked swab.

H3 — Step 2: Sample Lysis

Break the swab tip into the Lysis Buffer tube.

Vortex for approximately 30 seconds and briefly centrifuge or flick the tube to bring the liquid to the bottom.

H3 — Step 3: Sample Reconstitution

Transfer 20 μL of the processed sample into the Reconstitution Solution tube.

Vortex for approximately 10 seconds and briefly centrifuge.

The processed sample can then be used as the PCR template.

H3 — Step 4: Add Sample to Lyophilized PCR Reagent

Transfer 20 μL of the prepared sample into one tube of lyophilized PCR reagent.

Cap the tube securely, vortex for approximately 10 seconds, and briefly centrifuge.

H3 — Step 5: Real-Time PCR

Place the prepared reaction tube into the fluorescence PCR instrument and run the designated Canine Health Screening Panel (5-Plex) program.

The current instructions specify adding 20 μL of prepared sample to the lyophilized reagent and using the designated five-target PCR program.

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H2 — Real-Time Fluorescence PCR Detection

The Canine Health Screening Panel uses rapid real-time fluorescence PCR for molecular detection of the five canine pathogen targets.

The recommended workflow is:

Sample Collection → Lysis → Reconstitution → PCR Reaction → Real-Time Fluorescence Detection

The PCR program specified in the current instructions includes:

These conditions are taken directly from the current PD503 instructions.

H2 — Lyophilized PCR Reagent Format

The kit uses a lyophilized PCR reagent format, providing a convenient format for laboratory molecular testing.

H3 — Lyophilized Format Benefits

• Pre-formulated      PCR reagent

• Convenient laboratory      handling

• Reduced      reagent preparation

• Room-temperature      storage

• Suitable for      veterinary research workflows

The kit contains 10 Lysis Buffer tubes, 10 Reconstitution Solution tubes, 10 lyophilized reagent units and one bag of 8-tube strip caps.

H2 — Product Specifications

All of the above core specifications are based on the latest uploaded PD503 instruction sheet.

H2 — Required Instruments and Consumables

The product instructions specify the following equipment and laboratory consumables:

• T-8      Fluorescence PCR Pet Detection System

• Vortex Mixer

• Mini      Centrifuge

• Micropipette

• Pipette Tips

H2 — Result Interpretation

After PCR amplification, the fluorescence curve and threshold should be evaluated according to the product instructions.

The current PD503 instructions specify:

Ct ≤ 36.0 — Positive

Ct > 36.0 — Negative

The threshold should be adjusted based on the normal negative control and the best linearity of the amplification curves. A positive fluorescence curve should show a clear S-shaped amplification curve above the threshold.

Important: Result interpretation should follow the complete product instructions and appropriate laboratory quality-control procedures.

H2 — Applications

The Canine Health Screening Panel (5-Plex) qPCR Kit can support veterinary molecular research involving:

H3 — Canine Infectious Disease Research

Molecular research involving multiple canine pathogen targets.

H3 — Veterinary Laboratory Screening

Screening research involving canine infectious disease-associated pathogens.

H3 — Multiplex PCR Research

Research laboratories evaluating multi-target real-time PCR workflows.

H3 — Canine Health Research

Molecular research involving canine gastrointestinal, respiratory and systemic infectious disease-associated pathogens.

H2 — Key Features at a Glance

5-Pathogen Detection

CPV + CCoV + CDV + CAV-2 + Mycoplasma

Rapid Real-Time PCR

Rapid Real-Time Fluorescence PCR Method

Lyophilized Format

Convenient Lyophilized PCR Reagent

Multiple Sample Types

Anal / Oral / Nasal / Ocular Swabs

Room-Temperature Storage

0–20°C

12-Month Shelf Life

12 Months

10 Tests per Kit

10 Tests/Kit

H2 — Frequently Asked Questions

H3 — What pathogens does the Canine Health Screening Panel detect?

The kit detects five canine pathogen targets:

Canine Parvovirus, Canine Coronavirus, Canine Distemper Virus, Canine Adenovirus Type II and Mycoplasma.

H3 — How many pathogens are included in this canine PCR panel?

The product is a 5-Plex canine pathogen PCR panel containing five detection targets.

H3 — What sample types can be used?

The product instructions specify anal swabs and oral, nasal or ocular swab samples.

H3 — Is the PCR reagent lyophilized?

Yes. The kit uses a lyophilized PCR reagent format.

H3 — How many tests are included in one kit?

The package specification is 10 Tests/Kit.

H3 — What is the storage temperature?

The recommended storage temperature is 0–20°C.

H3 — What is the shelf life?

The stated shelf life is 12 months.

H3 — What PCR instrument is recommended?

The instructions specify the T-8 Fluorescence PCR Pet Detection System.

H3 — What is the positive Ct cutoff?

According to the current product instructions, a sample with Ct ≤ 36.0 is interpreted as positive, while Ct > 36.0 is interpreted as negative.

H3 — Is this product for clinical diagnosis?

No. The product is intended for veterinary research use only. It should not be presented as a product for human use, animal treatment or clinical diagnosis.

H2 — Canine PCR Panel for Veterinary Research

For laboratories looking for a Canine 5-Plex qPCR Kit, this product provides a convenient molecular testing solution covering five canine pathogen targets in one panel.

Canine Parvovirus

Canine Coronavirus

Canine Distemper Virus

Canine Adenovirus Type II

Mycoplasma

Combined with a rapid real-time fluorescence PCR method and lyophilized reagent format, the panel is designed for convenient use in veterinary molecular research laboratories.

H2 — Request a Quote

Looking for a Canine 5-Plex qPCR Kit for your veterinary laboratory?

Contact us for:

• Product      specifications

• Technical      documentation

• Evaluation      samples

• Bulk pricing

• Distributor      pricing

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