Feline Gastrointestinal 5-Plex qPCR Kit (FPV/FCoV/Giardia/Tritrichomonas/Cryptosporidium)

The Feline Gastrointestinal 5-Plex Pathogen qPCR Detection Kit (FVPC501) is an all-in-one multiplex real-time PCR panel designed for rapid identification of 5 major feline enteric pathogens: Feline Panleukopenia Virus (FPV), Feline Coronavirus (FCoV ), Giardia lamblia , Tritrichomonas foetus , and Cryptosporidium spp.

Utilizing advance lyophilization (freeze-drying) technology, all detection reagents are stabilized in solid pellets. This allows room temperature transit and storage (0–20°C) for 12 months, delivering fast results from anal swab samples.

Feline Gastrointestinal PCR Kit

Key Features & Benefits

5-in-1 Enteric Screening: Detects viral and protozoan intestinal pathogens simultaneously.
Room Temperature Storage: Lyophilized reagents remain stable at 0–20°C for up to 12 months.
Fast Anal Swab Extraction: Simple 2-step extraction protocol eliminates lengthy nucleic acid isolation steps.
10 Tests/Kit Pack Size: Economical multi-test specification for research laboratories and breeding facilities.
High Specificity: Prevents cross-reactivity between viral DNA/RNA and parasitic protozoa targets.

Kit Components

Component	Quantity	Main Function
Lysis Buffer	10 Tubes	Rapid lysis for fecal/anal swab matrix
Reconstitution Buffer	10 Tubes	Template buffer for neutralization
Lyophilized Reagents	10 Pouches	Freeze-dried 5-plex PCR reaction master mix
8-Strip Tube Caps	1 Pouch	Optical sealing caps for fluorescence detection

Sample Preparation & Rapid Extraction Protocol

Sample Collection: Collect an anal sample using a clean flocked anal swab.
Sample Lysis: Snap the swab head off into a tube of Lysis Buffer. Vortex for 30 seconds and gently flick/centrifuge liquid to the bottom.
Template Preparation: Pipette 20 µL of lysed liquid into a tube of Reconstitution Buffer. Vortex for 10 seconds and flick down. Use this solution as the amplification template.

Assay Procedure

Reconstitution:Add 20 µL of the prepared template into 1 tube of Lyophilized Reagent.
Cap & Mix: Cap with the 8-Strip Tube Cap, vortex for 10 seconds, and briefly centrifuge to drive liquid to the tube bottom and remove bubbles.
Loading: Place reaction tubes into the qPCR instrument with the black dot mark facing left.
Run Program: Choose the "FPV / FCoV / Giardia / T.foetus / Cryptosporidium" program and initiate.

Recommended Thermal Cycling Program

Stage	Temperature & Duration	Cycles	Signal Acquisition
Stage 1	55°C for 5 min	1	Off
Stage 2	95°C for 2 min	1	Off
Stage 3	95°C for 10 sec 58°C for 15 sec	40	Off Collect Fluorescence

Result Interpretation

Adjust baseline and fluorescence threshold line above negative control limits. A valid positive curve shows an obvious sigmoidal shape.

Positive Result (+): Test sample Ct value ≤ 36.0.
Negative Result (-): Test sample Ct value > 36.0 or no Ct value.

Precautions & Safety Handling

Research Use Only (RUO): Strictly for scientific research purposes.
Contamination Avoidance: Never re-open PCR tube caps post-amplification.
Workstation Sanitation: Disinfect surfaces with 10% bleach, 75% ethanol, or UV lighting.

Frequently Asked Questions (FAQ)

Q1: Why is testing for protozoa like Tritrichomonas foetus and Giardia important alongside FPV?

A1: Feline chronic diarrhea can stem from viral infections or protozoan parasites with identical symptoms. FVPC501 screens both viral and parasitic pathogens at once, saving diagnostic time.