Feline Infectious Peritonitis Virus (FIPV) qPCR Detection Kit (Real-Time PCR Method | Lyophilized)

The Feline Infectious Peritonitis Virus (FIPV) qPCR Detection Kit is a rapid molecular diagnostic reagent designed for the detection of FIP virus nucleic acid in feline ascites samples.

Based on real-time fluorescence PCR technology, this kit provides a fast, sensitive, and reliable method for researchers and veterinary diagnostic laboratories to identify FIPV infection.

The kit adopts a lyophilized reagent format, offering improved stability, convenient transportation, and simplified workflow compared with traditional liquid PCR reagents.

Key Features

Rapid FIP Virus Detection by Real-Time PCR

The kit uses fluorescence-based real-time PCR technology for specific amplification and detection of FIPV nucleic acid.

Key advantages:

• Fast      detection within approximately 1 hour

• High      analytical sensitivity and specificity

• Real-time      fluorescence monitoring

• Suitable for      veterinary molecular research

Lyophilized PCR Reagent Format

The freeze-dried (lyophilized) formulation provides:

• Enhanced      reagent stability

• Room      temperature transportation capability

• Reduced      cold-chain dependency

• Convenient      storage and handling

The kit can be stored at 0–20°C with a shelf life of 12 months.

Intended Use

Detection of Feline Infectious Peritonitis Virus (FIPV)

Feline infectious peritonitis (FIP) is a serious disease caused by feline coronavirus variants and is one of the important infectious diseases affecting domestic cats.

This kit is intended for:

• Veterinary      research laboratories

• Animal      disease monitoring studies

• Feline      infectious disease research

• Molecular      diagnostic method development

This product is intended for research use only (RUO). It is not intended for clinical diagnosis or therapeutic use.

Detection Principle

Real-Time Fluorescence PCR Technology

The kit detects FIPV nucleic acid through a fluorescence PCR amplification process.

Workflow:

Sample Collection
↓
Rapid Nucleic Acid Release
↓
PCR Amplification
↓
Real-Time Fluorescence Detection
↓
Result Analysis

The supplied reagents include:

• Lysis Buffer

• Reconstitution      Solution

• Lyophilized      PCR Reagents

• PCR Tube Caps

Sample Type

Compatible Sample

Recommended sample:

Ascites (abdominal fluid)

Sample preparation:

1. Add 20 μL      ascites sample into lysis buffer.

2. Perform rapid      nucleic acid release.

3. Transfer      processed sample into PCR reaction tube.

4. Run      fluorescence PCR detection.

Workflow

Simple Three-Step Detection Procedure

Step 1: Sample Preparation

Add ascites sample into the lysis solution for rapid template preparation.

Step 2: PCR Reaction Setup

Transfer processed sample into the lyophilized PCR reaction tube.

Step 3: Real-Time PCR Detection

Run the FIPV PCR program and analyze fluorescence curves.

The recommended amplification program includes:

• Reverse      transcription/activation step

• Denaturation      step

• Fluorescence      acquisition during amplification cycles

• 

Result Interpretation

Ct Value Analysis

Results are interpreted according to real-time PCR amplification curves.

• Ct ≤36.0:      Positive

• Ct >36.0:      Negative

A valid test requires appropriate positive and negative control performance.

Product Specifications

Why Choose Our FIPV qPCR Detection Kit?

Advantages Compared with Traditional Detection Methods

✔ Rapid Molecular Detection

PCR technology enables direct detection of viral nucleic acid.

✔ Convenient Workflow

Rapid sample processing reduces hands-on operation time.

✔ Stable Lyophilized Format

Suitable for international shipping and laboratory storage.

✔ Ready-to-Use PCR Solution

Simplifies workflow for veterinary laboratories and distributors.

Applications

Recommended Applications

• Veterinary      molecular research

• Feline      infectious disease studies

• Veterinary      laboratory testing development

• Animal health      research programs

• Companion      animal pathogen surveillance

FAQ

What sample type is required for FIPV PCR detection?

The recommended sample type is ascites (abdominal fluid) collected from cats.

Does this kit require RNA extraction?

The kit uses a rapid nucleic acid release procedure. Complex purification steps are not required for sample preparation.

Is this product for clinical diagnosis?

No. This product is intended for research use only (RUO) and is not intended for clinical diagnosis or treatment.

What is the storage condition?

The kit should be stored at 0–20°C and has a shelf life of 12 months.

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