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Efficient Exosomal RNA Extraction from 100–200 μL Exosome SamplesDesigned for concentrated RNA recovery from purified exosome suspensions using a silica column-based purification workflow.Product Overview
The Exosome RNA Extraction Kit is designed for the isolation and concentration of RNA from purified exosome suspensions. It is suitable for processing 100–200 μL of exosome sample and provides a simple column-based workflow for recovering exosomal RNA.
The purification system is designed to efficiently recover small RNA fragments while removing lipids, proteins and other contaminants. Following purification, exosomal RNA is eluted into a small volume of 30–50 μL RNase-free Water, providing a concentrated RNA preparation for downstream molecular biology applications.
The purified RNA can be used directly for RT-PCR, real-time RT-PCR, Northern blot and microarray analysis.
Key Features
Designed for 100–200 μL purified exosome suspensions
Efficient recovery of exosomal RNA, including small RNA fragments
Silica column-based purification
Lipids, proteins and other contaminants are removed during purification
No chloroform extraction required
RNA can be concentrated into 30–50 μL of RNase-free Water
Complete extraction workflow in approximately 1 hour
Suitable for RT-PCR, real-time RT-PCR, Northern blot and microarray analysis
How It Works
The kit uses a column-based RNA purification workflow:
Exosome Sample → Lysis → RNA Precipitation/Binding → Column Purification → Washing → Elution
For a standard 100 μL exosome suspension, Buffer TL is added to disrupt the sample matrix, followed by Buffer EX treatment and centrifugation. Ethanol is then added to the recovered supernatant before loading onto the nucleic acid purification column.
The column retains RNA while washing steps remove residual contaminants. After a final drying centrifugation, RNA is eluted using 30–50 μL RNase-free Water.
Exosome Suspension → Buffer Treatment → RNA Binding → Washing → Elution → Purified Exosomal RNA
Product Specifications
Parameter | Specification |
Product Name | Exosome RNA Extraction Kit |
Sample Type | Purified exosome suspension |
Recommended Sample Volume | 100–200 μL |
RNA Type | Exosomal RNA |
RNA Size | Includes small RNA fragments |
Purification Method | Silica column-based purification |
Elution Volume | 30–50 μL |
Workflow Time | Approximately 1 hour |
Chloroform Extraction | Not required |
Downstream Applications | RT-PCR, real-time RT-PCR, Northern blot, microarray analysis |
Package Size | 5 preps / 50 preps |
Intended Use | Research Use Only |
Exosome RNA Extraction Workflow
Step 1. Sample Treatment
Add 700 μL Buffer TL to 100 μL of purified exosome suspension and mix thoroughly.
For 200 μL of exosome suspension, use 1.4 mL Buffer TL according to the sample-volume ratio.
Step 2. Exosome Processing
Add Buffer EX according to the sample volume, mix thoroughly and centrifuge at 4°C, 12,000 rpm for 15 minutes.
Step 3. RNA Binding Preparation
Transfer the supernatant and add 2 volumes of absolute ethanol. Mix thoroughly.
Step 4. Column Purification
Load the mixture onto the nucleic acid purification column. Multiple loading cycles can be performed when processing larger sample volumes.
Step 5. Washing
Wash the column sequentially with Buffer WA and Buffer WBR to remove residual contaminants.
Step 6. Drying and Elution
Centrifuge at high speed to remove residual ethanol, then elute the purified RNA with 30–50 μL RNase-free Water.
Step 7. Downstream Applications
The purified exosomal RNA can be used immediately for downstream molecular biology experiments or stored below −70°C.
Applications
The purified exosomal RNA can be used for:
RT-PCR
Real-time RT-PCR
Northern blot
Microarray analysis
Exosome RNA research
Extracellular vesicle molecular biology research
Product Composition
50 Preps / Kit
Component | Quantity | Storage |
Buffer TL | 75 mL | 2–8°C |
Nucleic Acid Purification Columns | 50 | 0–30°C |
2 mL Collection Tubes | 50 | 0–30°C |
Buffer EX | 12 mL | 0–30°C |
Buffer WA, Concentrated | 15 mL | 0–30°C |
Buffer WBR, Concentrated | 10 mL | 0–30°C |
RNase-free Water | 2 mL × 2 | 0–30°C |
Instructions | 1 | — |
Package Options
Cat. No. | Preparation Size |
5202005 | 5 Preps |
5202050 | 50 Preps |
5-Prep Configuration
Component | Quantity |
Nucleic Acid Purification Columns | 5 |
2 mL Collection Tubes | 5 |
Buffer TL | 7.5 mL |
Buffer EX | 1.2 mL |
Buffer WA | 1.5 mL |
Buffer WBR | 1 mL |
RNase-free Water | 1.5 mL |
Instructions | 1 |
Storage and Shelf Life
Buffer TL: Store at 2–8°C.
Other components can be stored at 0–30°C.
When stored at 0–30°C, the kit can maintain its performance for 2 years.
Storage at 2–8°C can extend the product's usable life beyond two years according to the product specifications.
Who Can Use This Kit?
The Exosome RNA Extraction Kit is suitable for:
Molecular biology laboratories
Exosome and extracellular vesicle research laboratories
Cell biology research groups
Biotechnology companies
University and academic research laboratories
Laboratories performing RNA-based molecular assays
Research reagent distributors
Frequently Asked Questions
What sample volume is suitable for the Exosome RNA Extraction Kit?
The kit is designed for 100–200 μL of purified exosome suspension. For larger sample volumes, Buffer TL, Buffer EX and ethanol can be increased proportionally according to the sample volume.
What is the final RNA elution volume?
Purified exosomal RNA can be eluted in 30–50 μL RNase-free Water.
Does the kit require chloroform extraction?
No. The kit uses a column-based purification workflow and does not require chloroform extraction.
Can the kit recover small RNA from exosomes?
Yes. The purification column is designed to efficiently recover small RNA fragments from exosome samples.
What can the purified exosomal RNA be used for?
The purified RNA can be used for RT-PCR, real-time RT-PCR, Northern blot and microarray analysis.
Related RNA Extraction Products
For laboratories working with different biological sample types, Forest BioTech also provides RNA extraction solutions for:
Whole Blood Total RNA Extraction Kit
Micro Cells Total RNA Extraction Kit
Exosome RNA Extraction Kit
Need an Exosomal RNA Extraction Solution?
Forest BioTech provides RNA extraction kits for different biological sample types and research workflows.
Contact us for product specifications, sample evaluation, bulk orders and OEM/private-label opportunities.
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Research Use Only
For Research Use Only (RUO). This product is not intended for human or animal treatment or clinical diagnosis.
