Whole Blood Total RNA Extraction Kit is a rapid silica-column based purification system designed for the isolation of high-quality total RNA directly from whole blood samples.

Unlike conventional methods requiring red blood cell lysis and leukocyte separation, this kit enables direct RNA extraction from whole blood, significantly reducing sample handling time and minimizing RNA degradation.

Most importantly, the kit has been experimentally validated for RNA extraction from anticoagulated whole blood stored at -80°C for 75 days, making it highly suitable for:

• Biobanking samples

• Retrospective clinical studies

• Molecular diagnostics

• Gene expression analysis

• RT-PCR and RNA sequencing

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Key Advantages

⭐ Direct RNA Extraction from -80°C Frozen Whole Blood

Validated for whole blood samples stored at -80°C for up to 75 days.

High-quality RNA can be recovered directly from frozen anticoagulated whole blood without prior leukocyte isolation.

⭐ No Red Blood Cell Lysis Required

Traditional blood RNA extraction workflows require:

• Red blood cell lysis

• Leukocyte separation

• Multiple centrifugation steps

Our kit uses a direct lysis strategy, eliminating these labor-intensive procedures.

⭐ Compatible with Bone Marrow Samples

The kit is suitable for:

• Whole blood

• Frozen blood

• Bone marrow aspirates

making it a versatile solution for hematology and oncology research.

⭐ RT-PCR and RNA-Seq Ready

Purified RNA demonstrates:

• High purity

• Excellent integrity

• Strong RT-qPCR performance

• RNA sequencing compatibility

Ideal for downstream applications including:

• RT-PCR

• RT-qPCR

• RNA-Seq

• Gene expression profiling

⭐ Fast 20–40 Minute Workflow

RNA purification can be completed in only:

20–40 minutes

compared with:

60–120 minutes for traditional leukocyte-based methods.

Experimental Validation

The kit was evaluated using anticoagulated whole blood samples stored at -80°C for 75 days.

Experimental results demonstrated:

·        Successful RNA purification

·        High RNA purity

·        Excellent electrophoresis performance

·        Positive RT-qPCR amplification

·        Reliable downstream application compatibility

These results confirm the suitability of the kit for long-term frozen blood storage applications.

To evaluate long-term frozen blood compatibility, anticoagulated whole blood samples were stored at -80°C for 75 days.

Why Choose Our Whole Blood Total RNA Extraction Kit?

Comparison with Traditional Blood RNA Extraction Methods

 Applications

Molecular Biology Research

• Gene expression analysis

• Biomarker discovery

• Transcriptomics

Clinical Research

• Frozen biobank samples

• Longitudinal studies

• Clinical specimen analysis

Downstream Applications

• RT-PCR

• RT-qPCR

• RNA Sequencing

• cDNA synthesis

• NGS library preparation

Product Composition

Product Storage and Expiry Date

1.       Buffer L9 can be transported at room temperature, please store at 2~8 ℃ after receiving the product.

2.       Other reagents and components are stored at room temperature (0~30 ℃), the performance of the product can be maintained without significant change within two years; if the product is stored at 2~8 ℃, the validity period of the product can be extended to more than two years (the product stored at 2~8℃ should be restored to room temperature before use).

FAQ

Can froze blood samples be used directly?

Yes. The kit is validated for anticoagulated whole blood stored at -80°C.

Is red blood cell lysis required?

No. Direct RNA extraction from whole blood is supported.

Is leukocyte separation required?

No. The workflow eliminates the need for leukocyte isolation.

Can bone marrow samples be processed?

Yes. The kit is compatible with bone marrow specimens.

What downstream applications are supported?

RT-PCR, RT-qPCR, RNA-Seq, transcriptomics, viral RNA detection, fusion gene analysis, and gene expression profiling.

What is the extraction time?

Approximately 20–40 minutes.

Is the purified RNA suitable for RNA sequencing?

Yes. The purified RNA is fully compatible with RNA-Seq workflows.

What makes this kit different from conventional blood RNA extraction kits?

Unlike most commercial blood RNA extraction kits, this product has been experimentally validated for direct RNA extraction from anticoagulated whole blood stored at -80°C for 75 days, while maintaining excellent RT-qPCR performance and RNA quality.

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