Mob/ Whatsapp/Wechat: +86 138 806 76215 | Email: [email protected] / [email protected]
Direct RNA Extraction from -80°C Frozen Whole Blood or 500uL fresh bloodNo need for prior lysis to remove red blood cellsCompatible with Bone Marrow SamplesRT-PCR & RNA-Seq Read20-40 Minute WorkflowWhole Blood Total RNA Extraction Kit is a silica column-based purification system designed for total RNA isolation from fresh and -80°C frozen anticoagulated whole blood samples.
The streamlined workflow does not require a separate red blood cell (RBC) lysis or leukocyte separation step. The kit is also compatible with bone marrow samples and is designed to simplify RNA preparation for downstream molecular biology applications, including RT-PCR, RT-qPCR, gene expression analysis, and RNA sequencing (RNA-Seq).
RNA extraction from fresh and -80°C frozen anticoagulated whole blood
Evaluated using whole blood samples stored at -80°C for up to 75 days
No separate RBC lysis or leukocyte separation step
Silica column-based total RNA purification
Compatible with bone marrow samples
Typical extraction time: approximately 20–40 minutes
The kit has been evaluated for total RNA extraction from anticoagulated whole blood samples stored at -80°C for up to 75 days.
This feature may be useful for research laboratories working with archived frozen blood specimens, biobanking samples, and retrospective research collections.
The workflow does not require a separate red blood cell lysis or leukocyte isolation step before RNA purification. This helps simplify sample preparation and reduces the number of preliminary handling procedures.
The kit still requires sample processing and RNA purification according to the product protocol.
In addition to anticoagulated whole blood, the kit is also suitable for bone marrow specimens. Please refer to the product manual for the recommended sample preparation procedure.
Purified total RNA can be used for downstream molecular biology applications, including:
RT-PCR
RT-qPCR
Gene expression analysis
Transcriptomics research
RNA sequencing (RNA-Seq)
Suitability for each application depends on the RNA quality, quantity, and specific requirements of the downstream assay.
The typical RNA extraction workflow takes approximately 20–40 minutes, depending on sample type and processing conditions.
The kit was evaluated using anticoagulated whole blood samples stored at -80°C for 75 days to assess its performance in extracting total RNA from frozen blood specimens.
Experimental Conditions:
Sample Type: Anticoagulated whole blood
Storage Temperature: -80°C
Storage Duration: 75 days
RNA Quality Assessment: Agarose gel electrophoresis
Downstream Application Evaluation: RT-qPCR
Experimental Results:
The evaluation demonstrated successful total RNA purification from the frozen whole blood samples, with visible RNA bands on agarose gel electrophoresis and positive RT-qPCR amplification.
Figure Overview:
RNA Integrity Assessment: Agarose gel electrophoresis showing visible 28S and 18S rRNA bands.
Downstream RT-qPCR Evaluation: RT-qPCR amplification curves for β-actin, demonstrating successful amplification using the extracted RNA.
These results support the use of the kit for total RNA extraction from anticoagulated whole blood samples stored at -80°C for up to 75 days under the evaluated conditions.
The kit uses a silica column-based purification method to isolate total RNA from whole blood samples. The workflow eliminates the need for a separate RBC lysis or leukocyte isolation step.
Workflow Overview:
Prepare fresh or -80°C frozen anticoagulated whole blood samples.
Process the sample according to the kit protocol.
Perform RNA purification using the silica column.
Wash the column and elute the purified total RNA.
Use the extracted RNA for downstream applications such as RT-PCR, RT-qPCR, or RNA-Seq.
For detailed sample preparation steps, reagent volumes, and centrifugation conditions, please refer to the product manual.
Different blood RNA extraction products may use different sample preparation and purification procedures. The following table highlights the workflow characteristics of this kit compared with workflows that include separate leukocyte preparation.
| Feature | Workflows with Separate Leukocyte Preparation | Whole Blood Total RNA Extraction Kit |
|---|---|---|
| Separate RBC Lysis Step | May be required | No separate step required |
| Leukocyte Separation | May be required | No separate step required |
| Frozen Whole Blood Evaluation | Depends on the product and protocol | Evaluated at -80°C for 75 days |
| Purification Method | Varies by product | Silica column-based purification |
| Typical Processing Time | Depends on the workflow | Approximately 20–40 minutes |
Actual procedures and processing times vary among products, sample types, and laboratory protocols.
The Whole Blood Total RNA Extraction Kit is designed for research laboratories requiring total RNA purification from whole blood and bone marrow specimens.
Gene expression analysis
Transcriptomics research
Biomarker discovery
RNA-based molecular research
Archived frozen blood specimens
Retrospective research studies
Longitudinal sample analysis
Research involving bone marrow specimens
RT-PCR
RT-qPCR
cDNA synthesis
Gene expression profiling
RNA sequencing (RNA-Seq)
NGS library preparation, subject to RNA quality and quantity requirements
The Whole Blood Total RNA Extraction Kit is available in 5-prep and 50-prep packaging formats.
| Component | 5 Preps FR5201005 | 50 Preps FR5201050 |
|---|---|---|
| Spin Columns | 5 | 50 |
| 2 mL Collection Tubes | 5 | 50 |
| Buffer L9 | 6 mL | 55 mL |
| Buffer WA (Concentrate) | 1.9 mL | 12 mL |
| Buffer WBR (Concentrate) | 1.5 mL | 12 mL |
| RNase-free Water | 1.5 mL | 2 mL × 2 |
| Instructions | 1 copy | 1 copy |
Buffer L9: Can be transported at room temperature. Store at 2–8°C after receiving the product.
Other reagents and components: Store at 0–30°C. Product performance can be maintained without significant change for up to two years under the stated storage conditions.
Refrigerated storage: Storage at 2–8°C may extend the validity period beyond two years. Reagents stored at 2–8°C should be returned to room temperature before use.
Please refer to the product label and accompanying instructions for the applicable expiry date and storage requirements of each lot.
Yes. The kit has been evaluated using anticoagulated whole blood samples stored at -80°C for up to 75 days. Please follow the sample preparation procedure described in the product manual.
Please follow the sample handling instructions in the product manual. The protocol is designed to minimize sample handling and thawing time. Avoid leaving frozen blood samples at room temperature for extended periods before processing.
No separate RBC lysis or leukocyte separation step is required. Sample processing and RNA purification should still be performed according to the kit protocol.
Yes. The kit is also compatible with bone marrow specimens. Please refer to the product manual for the recommended sample preparation procedure.
Purified total RNA can be used for RT-PCR, RT-qPCR, gene expression analysis, transcriptomics, and RNA sequencing (RNA-Seq), subject to the RNA quality and quantity requirements of each application.
The typical RNA extraction workflow takes approximately 20–40 minutes, depending on the sample type and processing conditions.
The kit has been evaluated using anticoagulated whole blood samples stored at -80°C for up to 75 days. It also simplifies sample preparation by eliminating the need for a separate RBC lysis or leukocyte separation step.
Looking for an RNA extraction solution for fresh whole blood, archived frozen blood specimens, or bone marrow samples?
Contact us to discuss your sample type, storage conditions, and downstream application requirements. We can provide product specifications, protocol details, and evaluation options.
To help us recommend a suitable solution, please let us know:
Sample type: Whole blood or bone marrow
Sample storage condition: Fresh, -20°C, or -80°C
Sample volume per extraction
Downstream application: RT-PCR, RT-qPCR, or RNA-Seq
Estimated number of samples per month or year
Contact us for product specifications, protocol details, and evaluation options.
Discuss your research requirements with our team.
