Pigeon Gender Identification Kit (PCR Fluorescence Method,Lyophilized )Species-Specific CHD Gene

Accurate DNA-Based Pigeon Gender Identification

The Pigeon Gender Identification Kit (PCR Fluorescence Method, Lyophilized Type) is a real-time PCR assay specifically developed for accurate molecular sex determination of pigeons (Columba livia). The assay detects the pigeon-specific CHD (Chromo-Helicase-DNA Binding) gene using optimized primers and fluorescent probes designed according to pigeon genomic sequences.

Unlike conventional bird sex identification kits that rely on universal avian primers, this kit employs species-specific primer and probe design, significantly improving amplification specificity and analytical accuracy for pigeon samples.

The kit enables rapid identification of male (ZZ) and female (ZW) pigeons from feather, blood, or tissue samples, making it an ideal solution for racing pigeon breeders, commercial breeding farms, veterinary diagnostic laboratories, universities, and avian research institutes.

 Why Choose Our Pigeon Gender Identification Kit?

Species-Specific CHD Gene Design

Optimized primers and fluorescent probes are designed specifically according to the pigeon CHD gene sequence, providing superior specificity compared with universal avian assays.

High Analytical Accuracy

Species-specific amplification minimizes non-specific reactions and improves confidence in gender identification.

 Real-Time PCR Fluorescence Detection

Dual-fluorescence detection provides rapid, sensitive, and reliable DNA analysis.

 Lyophilized Ready-to-Use Reagents

Freeze-dried reagents simplify transportation, improve storage stability, and eliminate the need for cold-chain shipping.

Rapid DNA Release

Simple nucleic acid release protocol eliminates conventional DNA extraction procedures for feather samples.

Fast Workflow

Complete testing can be completed within approximately 60 minutes.

Molecular Principle

Birds possess a ZW sex chromosome system, in which:

The CHD gene is located on both the Z and W chromosomes but contains sequence differences that allow molecular discrimination between male and female birds.

Although many bird species utilize CHD gene-based sex determination, the nucleotide sequence of the CHD gene differs among species. Our assay has been specifically optimized using pigeon-derived CHD gene sequences to design highly specific primers and fluorescent probes, ensuring superior analytical performance for pigeon DNA.

This species-specific assay significantly reduces non-specific amplification and improves detection accuracy compared with universal bird gender identification methods.

Key Features

• Species-specific CHD gene primers and probes

• Designed exclusively for pigeon DNA detection

• High specificity and sensitivity

• Real-time PCR fluorescence technology

• Lyophilized ready-to-use reagents

• Room-temperature transportation

• Direct feather DNA release

• Compatible with blood and tissue DNA

• Excellent repeatability and reproducibility

• Research Use Only (RUO)

Applications

The kit is suitable for:

• Racing pigeon breeding

• Homing pigeon breeding

• Fancy pigeon breeding

• Commercial pigeon farms

• Veterinary diagnostic laboratories

• Universities

• Molecular biology laboratories

• Avian genetics research

• Wildlife conservation programs

 Compatible Sample Types

Validated sample types include:

• Feather pulp (Recommended)

• Whole blood

• Fresh tissue

• Purified genomic DNA

Kit Contents

Specification: 48 Tests/Kit

 Storage and Shelf Life

• Store at 0–25°C

• Shelf life: 12 months

• Lyophilized reagents provide excellent transportation stability without dry ice.

Workflow

1. Collect feather, blood, or tissue samples.

2. Release genomic DNA using the supplied nucleic acid release reagent.

3. Reconstitute the lyophilized PCR reagents.

4. Add the DNA sample to the PCR reaction tube.

5. Perform real-time PCR amplification.

6. Interpret fluorescence signals to determine pigeon gender.

Advantages Over Universal Bird Gender Identification Kits

Frequently Asked Questions

 Why does this kit use species-specific primers?

Although the CHD gene is widely used for avian gender identification, its nucleotide sequence varies among different bird species. Our primers and probes are specifically designed according to the pigeon CHD gene sequence, improving amplification specificity and reducing the possibility of non-specific reactions.

 Can feather samples be used?

Yes. Feather pulp provides sufficient genomic DNA for reliable real-time PCR detection and is the recommended sample type.

What fluorescence channels are required?

The assay is compatible with most real-time PCR instruments equipped with FAM and VIC (HEX) fluorescence channels.

 Is this kit suitable for racing pigeons?

Yes. It is widely applicable to racing pigeons, breeding pigeons, ornamental pigeons, and research applications.

Related Products

• Parrot Gender Identification Kit

• Canary Gender Identification Kit

• Bird DNA Extraction Kit

• Universal DNA Release Reagent

• Lyophilized PCR Reagent Series

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