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RNA EXTRACTION KIT Whole Blood Total RNA Extraction KitDirect RNA Extraction from Fresh and -80°C Frozen Whole Blood |
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The Whole Blood Total RNA Extraction Kit is a silica-column-based purification system designed for total RNA isolation directly from whole blood samples. Unlike conventional workflows that may require red blood cell lysis and leukocyte separation, this kit supports direct whole blood RNA extraction, simplifying sample preparation. The kit has been experimentally evaluated using anticoagulated whole blood stored at -80°C for 75 days. 20–40 min workflow No RBC lysis step 75-day frozen sample evaluation |
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01. Direct Whole Blood ExtractionNo separate red blood cell lysis or leukocyte separation step is required by the kit workflow. |
02. Frozen Blood EvaluationExperimentally evaluated with anticoagulated whole blood stored at -80°C for 75 days. |
03. Bone Marrow CompatibilitySuitable for whole blood and compatible bone marrow specimens. |
04. Downstream RNA ApplicationsDesigned for RNA applications including RT-PCR, RT-qPCR and RNA sequencing workflows. |
The silica-column-based workflow takes approximately 20–40 minutes, according to the product protocol.
The kit simplifies whole blood RNA preparation by combining direct sample processing with silica-column-based RNA purification.
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STEP 1 Blood SampleFresh or frozen anticoagulated whole blood |
STEP 2 Direct ProcessingFollow the kit protocol without separate RBC lysis |
STEP 3 RNA PurificationSilica-column-based purification |
STEP 4 Purified RNAFor downstream molecular applications |
Follow the product manual for sample input, processing conditions, centrifugation settings and elution steps.
EXPERIMENTAL EVIDENCE
The kit was evaluated using anticoagulated whole blood samples stored at -80°C for 75 days.
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Evaluation Highlights
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Figure overview
The figure presents RNA integrity assessment and downstream RT-qPCR evaluation using anticoagulated whole blood stored at -80°C for 75 days.
Interpret experimental findings in the context of the samples, protocol and conditions tested. Results may vary with sample characteristics and handling.
Read the Direct RNA Extraction from Frozen Whole Blood Protocol
Read the High-Quality RNA Extraction from -80°C Frozen Whole Blood Guide
The following table summarizes general workflow differences. Actual steps and processing times depend on the method and sample type used.
| Feature | Conventional Leukocyte-Based Workflow | Whole Blood Total RNA Extraction Kit |
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| Sample preparation | May involve RBC lysis and leukocyte separation | Direct whole blood processing according to the kit protocol |
| Workflow time | Depends on the method and preparation steps | Approximately 20–40 minutes |
| Frozen blood evaluation | Depends on the method and its validation | Evaluated with anticoagulated whole blood stored at -80°C for 75 days |
| Downstream applications | Depends on RNA quality and the workflow | RT-PCR, RT-qPCR and RNA sequencing workflows |
Molecular Biology Research
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Clinical and Biobank Research
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Downstream RNA Analysis
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Gene Expression Studies
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| Component | 5 Preps | 50 Preps |
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| Spin Columns | 5 | 50 |
| 2 mL Collection Tubes | 5 | 50 |
| Buffer L9 | 6 mL | 55 mL |
| Buffer WA (concentrate) | 1.9 mL | 12 mL |
| Buffer WBR (concentrate) | 1.5 mL | 12 mL |
| RNase-free Water | 1.5 mL | 2 mL × 2 |
| Instructions | 1 copy | 1 copy |
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5 Preps Catalog No.: FR5201005 |
50 Preps Catalog No.: FR5201050 |
Buffer L9: Can be transported at room temperature. Store at 2–8°C after receiving the product.
Other reagents and components: Store at 0–30°C. Product performance can be maintained without significant change for two years under the stated conditions. Storage at 2–8°C may extend the validity period beyond two years. Allow components stored at 2–8°C to return to room temperature before use.
The kit has been experimentally evaluated using anticoagulated whole blood stored at -80°C for 75 days. Follow the product protocol and consider your sample type and handling conditions.
Follow the kit manual's direct extraction procedure for frozen blood samples. Sample handling should be consistent with the validated protocol.
No separate red blood cell lysis step is required by the kit workflow.
The kit supports direct whole blood processing without a separate leukocyte separation step.
The kit is compatible with bone marrow specimens. Please consult the manual for sample input and processing requirements.
The kit is intended for RNA research workflows such as RT-PCR, RT-qPCR, RNA sequencing and gene expression analysis, subject to downstream assay requirements.
The workflow takes approximately 20–40 minutes according to the product information.
RNA sequencing is a stated downstream application. Suitability for a specific library preparation workflow should be confirmed using the relevant RNA quality and input requirements.
Technical Guide 1
High-Quality RNA Extraction from -80°C Frozen Whole Blood Guide
Technical Guide 2
Direct RNA Extraction from Frozen Whole Blood Protocol
Contact our team for product specifications, protocol details and quotation requests for your research requirements.
For Research Use Only (RUO). Not intended for diagnostic procedures.
