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Canine Respiratory Pathogen Panel (6-Plex) qPCR Kit | Real-Time PCR

Canine Respiratory Pathogen Panel (6-Plex) qPCR Kit | Real-Time PCR

  • highlight iconSimultaneous detection of Canine Distemper/ Parainfluenza/Adenovirus II Bordetella/Mycoplasma ,Chlamydia
  • highlight iconDetection by Real-Time PCR finish detection in 1 hour
  • highlight iconLyophilized PCR Reagent Format
  • highlight iconDirect PCR using lysis solution for rapid template preparation
  • highlight iconResearch Use Only

The Canine Respiratory Pathogen Panel (6-Plex) qPCR Kit (FVPD601)is designed for molecular detection of six canine respiratory targets:

  • Canine Distemper Virus

  • Canine Parainfluenza Virus

  • Canine Adenovirus Type II

  • Bordetella bronchiseptica

  • Mycoplasma

  • Chlamydia

The kit uses a lyophilized rapid PCR formatand is designed for oral, nasal, and ocular mucosal swab samples.

 Canine Respiratory Pathogen Panel (6-Plex) qPCR Kit





Six-Target Canine Respiratory Detection

The panel combines six respiratory targets into one molecular testing workflow.

Target

Detection

Canine Distemper Virus

Molecular detection

Canine Parainfluenza Virus

Molecular detection

Canine Adenovirus Type II

Molecular detection

Bordetella bronchiseptica

Molecular detection

Mycoplasma

Molecular detection

Chlamydia

Molecular detection

Canine Respiratory Pathogen Panel (6-Plex) qPCR Kit

This format can help research laboratories perform broader molecular screening when multiple respiratory targets need to be evaluated.




Oral, Nasal and Ocular Swab Samples

The product instructions specify oral, nasal, and ocular mucosal swabs.

Sample Processing

  1. Collect the oral, nasal, and ocular mucosal swab.

  2. Place the swab head into the lysis solution.

  3. Vortex for 30 seconds.

  4. Transfer 20 μL into the reconstitution solution.

  5. Vortex for 10 seconds.

  6. Transfer 20 μL into the lyophilized PCR reagent.

  7. Run the fluorescence PCR program.

 




Lyophilized Canine Respiratory PCR Kit

The kit includes:

  • 10 lysis solution tubes

  • 10 reconstitution solution tubes

  • 10 lyophilized reagent bags

  • 1 bag of 8-strip tube caps

Package Size: 10 Tests/Kit

Storage is specified at 0–20°C, with a 12-month shelf life.




Rapid Real-Time Fluorescence PCR

The assay uses the following PCR program:

Step

Temperature

Time

Cycles

1

55°C

5 min

1

2

95°C

2 min

1

3

95°C

10 sec

40

4

58°C

15 sec

40

Fluorescence is collected during amplification.

 




Ct-Based Result Interpretation

According to the product instructions:

  • Ct ≤ 36.0 — Positive

  • Ct > 36.0 — Negative

The threshold should be adjusted according to the negative control and amplification curve characteristics. If the positive and negative controls do not meet the specified requirements, the result is considered invalid and testing should be repeated.

Canine Respiratory Pathogen Panel (6-Plex) qPCR Kit


Key Features


6-Plex Canine Respiratory Panel

Six canine respiratory targets are included in one molecular testing workflow.

Lyophilized PCR Format

The PCR reagent is supplied in lyophilized format.

Oral, Nasal and Ocular Swab Compatible

Designed for oral, nasal and ocular mucosal swab samples.

Rapid Fluorescence PCR

Uses a rapid real-time fluorescence PCR workflow with 40 amplification cycles.


Product Specifications

Specification

Details

Product Name

Canine Respiratory Pathogen   Panel (6-Plex) qPCR Kit

Method

Rapid Real-Time Fluorescence   PCR

Format

Lyophilized

Sample Type

Oral / Nasal / Ocular Swab

Targets

6

Package

10 Tests/Kit

Storage

0–20°C

Shelf Life

12 Months

Intended Use

Veterinary research


Related Products 


Canine Distemper Virus (CDV) qPCR Detection Kit(FVPD102)

Canine Parvovirus & Coronavirus Duplex qPCR Kit(FVPD201)

Canine Respiratory Pathogen Panel (5-Plex) qPCR Kit(FVPD501)

Canine Gastrointestinal Pathogen Panel (5-Plex) qPCR Kit(FVPD502)



Frequently Asked Questions


What pathogens does the canine respiratory PCR panel detect?

The panel detects Canine Distemper Virus, Canine Parainfluenza Virus, Canine Adenovirus Type II, Bordetella bronchiseptica, Mycoplasma, and Chlamydia.


What sample type is required?

The recommended sample is an oral, nasal, and ocular mucosal swab.


Is this a lyophilized PCR kit?

Yes. The PCR reagent is supplied in lyophilized format.


How many tests are included in one kit?

Each kit contains 10 tests.


What Ct value is considered positive?

According to the product instructions, Ct ≤36.0 is interpreted as positive, while Ct >36.0 is interpreted as negative.

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Canine Respiratory Pathogen Panel (6-Plex) qPCR Kit

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