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Feline Panleukopenia & Coronavirus Duplex qPCR Kit | Real-Time PCR

Feline Panleukopenia & Coronavirus Duplex qPCR Kit | Real-Time PCR

  • highlight iconSimultaneous detection of Feline Panleukopenia Virus & Feline Coronavirus
  • highlight iconDetection by Real-Time PCR finish detection in 1 hour
  • highlight iconLyophilized PCR Reagent Format
  • highlight iconDirect PCR using lysis solution for rapid template preparation
  • highlight iconResearch Use Only

The Feline Panleukopenia & Coronavirus Duplex qPCR Kit(FVPC201) is designed for molecular detection of two important feline viral targets in a single testing workflow:

  • Feline Panleukopenia Virus

  • Feline Coronavirus

The kit uses a lyophilized rapid PCR formatand is designed for anal swab samples.

Feline Panleukopenia Coronavirus  qPCR Kit

 

 


Two-Target Feline Viral Detection

The duplex configuration allows simultaneous molecular testing for:

Target

Detection

Feline Panleukopenia Virus

Molecular detection

Feline Coronavirus

Molecular detection

Feline Panleukopenia Coronavirus  qPCR Kit

This provides laboratories with a focused molecular testing option when both targets are relevant to the research workflow.




Anal Swab Sample Testing

The product instructions specify anal swabs as the sample type.

The sample preparation workflow includes:

  1. Collect an anal swab using a new flocked swab.

  2. Place the swab head into the lysis solution.

  3. Vortex for 30 seconds.

  4. Transfer 20 μL of processed sample into the reconstitution      solution.

  5. Vortex for 10 seconds.

  6. Transfer 20 μL into the lyophilized PCR reagent.

  7. Run the fluorescence PCR program.


Lyophilized Duplex PCR Kit

Each kit contains:

  • 10 lysis solution tubes

  • 10 reconstitution solution tubes

  • 10 lyophilized PCR reagent bags

  • 8-strip tube caps

Package: 10 Tests/Kit

The specified storage condition is 0–20°C, with a 12-month shelf life.


Real-Time Fluorescence PCR

The PC201 assay uses:

55°C for 5 minutes → 95°C for 2 minutes → 95°C for 10 seconds / 58°C for 15 seconds × 40 cycles

Fluorescence is collected during amplification.



Ct-Based Result Interpretation

According to the product instructions:

Ct ≤ 36.0 = Positive

Ct > 36.0 = Negative

If the positive and negative controls do not meet the specified conditions, the test result is considered invalid and testing should be repeated.

Feline Panleukopenia Coronavirus  qPCR Kit



Key Features


Duplex Molecular Detection

Two feline viral targets are included in one PCR workflow.

Lyophilized Format

The PCR reagent is supplied in a convenient lyophilized format.

Anal Swab Compatible

The kit is designed for anal swab samples.

10-Test Configuration

Each package contains 10 tests.



Product Specifications


Specification

Details

Product Name

Feline Panleukopenia &   Coronavirus Duplex qPCR Kit

Method

Rapid Real-Time Fluorescence   PCR

Format

Lyophilized

Sample Type

Anal Swab

Targets

2

Package

10 Tests/Kit

Storage

0–20°C

Shelf Life

12 Months

Intended Use

Veterinary research




Frequently Asked Questions


What does this feline duplex PCR kit detect?

It detects feline panleukopenia virus and feline coronavirus.


What sample type is required?

The recommended sample type is an anal swab.


Is the reagent lyophilized?

Yes. The PCR reagent is supplied in lyophilized format.


How many tests are included?

10 tests per kit.


What is the positive Ct threshold?

According to the product instructions, Ct ≤36.0 is interpreted as positive.

 

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 Feline Panleukopenia Coronavirus  qPCR Kit


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