Feline Respiratory Pathogen Panel (6-Plex) qPCR Kit | Real-Time PCR

The Feline Respiratory Pathogen Panel (6-Plex) qPCR Kit（FVPC602） is a lyophilized rapid PCR reagent designed for the molecular detection of six feline respiratory pathogens.

The panel targets:

• Feline Herpesvirus

• Feline Calicivirus

• Feline Mycoplasma

• Feline Chlamydia

• Bordetella bronchiseptica

• Feline Influenza

The kit is designed for testing of oral, nasal, and ocular swab samples using a fluorescence PCR instrument.

Feline Herpesvirus | Calicivirus | Mycoplasma | Chlamydia | Bordetella | Influenza

Six-Pathogen Feline Respiratory Detection

Respiratory disease in cats can involve multiple infectious agents with overlapping clinical signs. A multi-target molecular assay can provide a broader testing approach than testing for a single pathogen alone.

This panel combines six targets in one testing solution:

This configuration makes the kit suitable for research laboratories, veterinary diagnostic research, and molecular testing workflows

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Note: The product is intended for basic research and is not intended for human or animal treatment or clinical diagnosis, according to the product instructions.

Sample Type: Oral, Nasal and Ocular Swabs

The recommended sample type is a combined oral, nasal, and ocular mucosal swab sample.

The supplied instructions describe a direct sample-processing workflow:

1. Collect the oral, ocular, and nasal mucosal swab.

2. Place the swab head into the lysis solution.

3. Vortex for 30 seconds.

4. Transfer 20 μL of the processed sample into the reconstitution      solution.

5. Vortex for 10 seconds.

6. Transfer 20 μL of the processed sample into the lyophilized      PCR reagent.

7. Run the fluorescence PCR program.

Lyophilized qPCR Format for Convenient Storage

The kit uses a lyophilized PCR reagent format.

Each kit contains:

• 10 lysis solution tubes

• 10 reconstitution solution tubes

• 10 lyophilized reagent bags

• 1 bag of 8-strip tube caps

Package Size: 10 tests/kit

The product instructions specify storage at 0–20°C with a 12-month shelf life.

Real-Time Fluorescence PCR Workflow

The PC602 assay uses the following PCR program:

Fluorescence is collected during amplification.

Ct-Based Result Interpretation

According to the product instructions:

• Ct ≤ 36.0: Positive

• Ct > 36.0: Negative

The fluorescence threshold should be adjusted based on the negative control and the best linearity of the amplification curves. A positive amplification curve should show a clear S-shaped curve above the threshold.

Key Features

6-Plex Feline Respiratory Pathogen Panel

Six respiratory targets are included in one molecular testing panel.

Lyophilized PCR Reagent

The PCR reagent is supplied in a lyophilized format.

Oral, Nasal and Ocular Swab Samples

Designed for testing of oral, nasal and ocular mucosal swab samples.

Rapid Fluorescence PCR Workflow

The assay uses a rapid fluorescence PCR program with 40 amplification cycles.

Product Specifications

Frequently Asked Questions

What pathogens does this feline respiratory PCR panel detect?

The panel detects Feline Herpesvirus, Feline Calicivirus, Feline Mycoplasma, Feline Chlamydia, Bordetella bronchiseptica, and Feline Influenza.

What sample type is required?

The recommended sample is an oral, nasal, and ocular mucosal swab.

Is the PCR reagent lyophilized?

Yes. The PCR reagent is supplied in lyophilized format.

How many tests are included in one kit?

Each kit contains 10 tests.

What is the storage temperature?

The product instructions specify storage at 0–20°C.

What Ct value is considered positive?

According to the product instructions, samples with Ct ≤36.0 are interpreted as positive, while Ct >36.0 is interpreted as negative.

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