Feline Anemia Pathogen Panel (4-Plex) qPCR Kit |Real-Time PCR

Product Description

The Feline Anemia Pathogen Panel (4-Plex) qPCR Kit (FVPC401) is a rapid molecular testing reagent designed for the detection of four feline pathogen nucleic acid targets associated with feline anemia and related infectious disease research.

The panel targets Bartonella henselae, hemotropic Mycoplasma, Feline Leukemia Virus (FeLV), and Feline Immunodeficiency Virus (FIV) using fresh EDTA-anticoagulated whole blood samples.

Based on rapid PCR and real-time fluorescence detection technology, PC401 provides a streamlined workflow for veterinary molecular research and feline infectious disease studies.

The kit uses a lyophilized PCR reagent format together with lysis and reconstitution solutions for rapid sample preparation.

For Veterinary Research Use Only. Not for use in human or animal clinical diagnosis, treatment, or prevention.

 Key Features

4-Target Feline Anemia Pathogen Detection

The Feline Anemia Pathogen Panel (4-Plex) qPCR Kit (FVPC401)  detects four feline pathogen targets:

• Bartonella      henselae

• Hemotropic      Mycoplasma

• Feline      Leukemia Virus (FeLV)

• Feline      Immunodeficiency Virus (FIV)

  

This multi-target format provides a practical molecular research solution for studying infectious agents associated with feline anemia and related feline health conditions.

Rapid Real-Time Fluorescence PCR

FVPC401 uses a rapid fluorescence PCR workflow.

The supplied PCR program includes:

• 55°C for 5      minutes

• 95°C for 2      minutes

• 40 cycles of:

• 95°C for 10       seconds

• 58°C for 15       seconds with fluorescence acquisition

Lyophilized PCR Reagent Format

FVPC401 uses a lyophilized PCR reagent format for convenient laboratory handling.

Each kit contains:

• 5 Lysis      Buffer tubes

• 5      Reconstitution Solution tubes

• 5 Lyophilized      PCR Reagent bags

• 8-Strip Tube      Caps

Package size:

5 Tests/Kit

Storage:

0–20°C

Shelf life:

12 Months

Intended Use

Veterinary Molecular Research

FVPC401 is intended for molecular research involving feline infectious diseases and pathogen-associated anemia studies.

Potential applications include:

• Feline anemia      pathogen research

• Feline      infectious disease research

• Veterinary      molecular biology research

• Companion      animal pathogen studies

• Veterinary      laboratory method development

• Multi-pathogen      screening research

Research Use Only (RUO).

This product is not intended for human or animal clinical diagnosis, treatment, or prevention.

Detection Targets

Four Feline Pathogen Targets

Sample Type

Fresh EDTA-Anticoagulated Whole Blood

PC401 uses fresh EDTA-anticoagulated whole blood as the sample type.

Sample Preparation

1. Collect fresh      EDTA-anticoagulated whole blood.

2. Transfer 20      μL whole blood into the lysis buffer.

3. Vortex for 30      seconds.

4. Transfer 20      μL processed sample into the reconstitution solution.

5. Vortex for 10      seconds.

6. Use the      processed sample as the PCR template.

The sample preparation procedure follows the supplied PC401 instructions.

 Simple & Rapid PCR Workflow

Step 1 – Blood Sample Collection

Collect fresh EDTA-anticoagulated whole blood.

↓

Step 2 – Sample Lysis

Add 20 μL blood sample to the lysis buffer and vortex for 30 seconds.

↓

Step 3 – Reconstitution

Transfer 20 μL processed sample to the reconstitution solution.

↓

Step 4 – PCR Setup

Transfer 20 μL processed sample to the lyophilized PCR reagent.

↓

Step 5 – Real-Time Fluorescence PCR

Run the FVPC401 PCR program.

↓

Step 6 – Result Analysis

Analyze fluorescence amplification curves and Ct values.

Result Interpretation

Ct Value Analysis

Results are interpreted based on real-time fluorescence PCR amplification curves.

• Ct ≤ 36.0:      Positive

• Ct > 36.0:      Negative

Threshold and baseline settings should be adjusted according to the amplification curves and control performance.

Product Specifications

Why Choose the Feline Anemia Pathogen Panel?

Four-Target Molecular Testing

FVPC401 combines four feline pathogen targets in one molecular research panel, providing a broader testing approach than single-target PCR assays.

Whole Blood-Compatible Workflow

The supplied protocol is designed for fresh EDTA-anticoagulated whole blood, allowing direct sample processing through the supplied lysis and reconstitution steps.

Rapid PCR Workflow

The streamlined sample preparation and fluorescence PCR protocol are designed to reduce hands-on processing steps.

Lyophilized PCR Reagents

The lyophilized reagent format provides convenient handling and laboratory storage.

Applications

FVPC401 can support:

• Feline anemia      pathogen research

• Feline      infectious disease research

• Veterinary      molecular diagnostics research

• Companion      animal pathogen studies

• Veterinary      laboratory method development

• Multi-pathogen      screening research

• Feline health      research programs

 FAQ

What pathogens does the FVPC401 feline anemia panel detect?

FVPC401 detects:

Bartonella henselae, Hemotropic Mycoplasma, Feline Leukemia Virus (FeLV), and Feline Immunodeficiency Virus (FIV).

What sample is required for FVPC401?

The supplied instructions specify fresh EDTA-anticoagulated whole blood.

Does FVPC401 require conventional DNA extraction?

The supplied workflow uses lysis and reconstitution solutions for rapid sample preparation before PCR rather than a conventional column or magnetic-bead nucleic acid purification procedure.

What is the Ct cutoff?

According to the supplied instructions:

• Ct ≤36.0 =      Positive

• Ct >36.0 =      Negative

How many tests are included in one FVPC401 kit?

PC401 contains 5 tests per kit.

What is the storage condition?

Store at 0–20°C. The stated shelf life is 12 months.

Is FVPC401 intended for clinical diagnosis?

FVPC401 is intended for veterinary research use only and is not intended for clinical diagnosis, treatment, or prevention.

Related Feline Veterinary PCR Kits

• Feline      Infectious Peritonitis Virus (FIPV) qPCR Detection Kit

• Feline      Panleukopenia Virus (FPV) qPCR Detection Kit

• Feline      Herpesvirus & Calicivirus Duplex qPCR Kit

• Feline      Respiratory Pathogen Panel (6-Plex) qPCR Kit

• Feline      Gastrointestinal Pathogen Panel (5-Plex) qPCR Kit

• Feline      Diarrhea Pathogen Panel (5-Plex) qPCR Kit

• Feline HCM      Three-Variant Genotyping Kit

• Feline PKD      Variant Detection Kit

 Looking for a Feline Veterinary PCR Kit Supplier?

Forest BioTech supplies feline and canine PCR/qPCR kits for veterinary research laboratories, biotechnology companies, distributors, and OEM/private-label projects.

If you are interested in:

• Feline anemia      PCR panels

• Feline      infectious disease qPCR kits

• Veterinary      pathogen detection panels

• Feline      genetic testing kits

• OEM/private-label      veterinary PCR products

Contact Forest BioTech to request product specifications, evaluation samples, distributor information, or OEM/private-label cooperation.

CTA

Request Product Information

Request Evaluation Sample

Ask About OEM / Private Label