Feline Respiratory 5-Plex qPCR Kit (FHV-1/FCV/Mycoplasma/Chlamydia/B.bronchiseptica)

The Feline Respiratory 5-Plex Pathogen qPCR Detection Kit (FVPC502) is a comprehensive, multiplex molecular panel designed for the direct and simultaneous detection of 5 major feline respiratory pathogens: Feline Herpesvirus-1 (FHV-1), Feline Calicivirus (FCV), Feline Mycoplasma, Chlamydia felis, and Bordetella bronchiseptica Featuring proprietary lyophilization technology, all reaction enzymes, primers, and probes are dried inside the tubes, supporting room temperature storage and transportation at 0–20°C for 12 months.

Key Features & Benefits

• Comprehensive      5-in-1 Panel: Screens for      viral, mycoplasmal, chlamydial, and bacterial respiratory pathogens in a      single workflow.

• Room      Temperature Stability: Freeze-dried      format allows storage at 0–20°C for up to 12 months.

• 10-Test Pack      Size: Ideal for high-throughput      screening and panel evaluation.

• High      Efficiency: Employs a rapid 2-step      extraction protocol without column or magnetic bead purification.

• Broad Sample      Compatibility: Validated      for oral, nasal, and conjunctival mucosal swabs.

Kit Components

Sample Preparation & Rapid Extraction Protocol

1. Sample      Collection: Use a sterile flocked      swab to collect ocular, nasal, or oral mucosal secretions from the      feline subject.

2. Rapid Lysis: Snap off the swab head into a tube of Lysis      Buffer. Vortex for 30 seconds, then flick or spin down liquid      to the bottom.

3. Template      Neutralization: Transfer 20      µL of the lysate into a tube of Reconstitution Buffer. Vortex      for 10 seconds and flick liquid down. The resulting solution is used      directly as the amplification template.

Assay Procedure

1. Reconstitution: Dispense 20 µL of the processed sample      template into 1 tube of Lyophilized Reagent.

2. Mix &      Cap: Cap tightly with an 8-Strip      Cap, vortex for 10 seconds, and centrifuge briefly to clear      bubbles and drop liquid to the bottom.

3. Loading: Load the strip into the fluorescent PCR      instrument with the black dot facing left.

4. Execution: Select the pre-configured program: "FHV-1      / FCV / F.Mycoplasma / C.felis / B.bronchiseptica" and start      running.

Recommended Thermal Cycling Program

Result Interpretation

Adjust baseline and fluorescence thresholds. An optimal threshold line sits above the highest point of a normal negative control curve. A valid positive reaction exhibits a distinct S-shaped amplification curve.

• Positive      Result (+): Test sample Ct value      ≤ 36.0.

• Negative      Result (-): Test sample Ct value      > 36.0 or no Ct detected.

Precautions & Safety Handling

1. Research Use      Only (RUO): Restricted to scientific      research; not for human/animal clinical diagnosis.

2. Contamination      Avoidance: Perform steps in      designated clean rooms; avoid opening amplification tubes post-run.

3. Sanitation: Disinfect workstations with 10% bleach or 75%      ethanol regularly.

Related Products & Topic Clusters

Related Veterinary qPCR Kits

• Feline      Gastrointestinal 5-Plex qPCR Detection Kit (FVPC501)

• Feline      Herpesvirus / Calicivirus 2-Plex Kit (FVPC203)

Frequently Asked Questions (FAQ)

Q1: Can bacterial and viral targets be amplified at the same time in FVPC502?

A1: Yes. The enzymatic master mix in FVPC502 is specifically optimized to co-amplify bacterial, mycoplasmal, and viral genomic targets without cross-channel interference.

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